Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition

Journal: Metabolites

Article Title: Hepatic CYP3A4 Enzyme Compensatively Maintains Endogenous Geranylgeranoic Acid Levels in MAOB -Knockout Human Hepatoma Cells

doi: 10.3390/metabo12020140

Figure Lengend Snippet: Cytochrome P450 enzyme inhibitors-induced downregulation of cellular GGA levels in Hep3B/ MAOB -KO cells. Dose-dependent changes of endogenous GGA level in Hep3B/ MAOB -KO cells (closed circle) and Hep3B/ MAOB -WT cells (open circle) after treatment with 0–100 μM of ABT ( A ), BG ( B ) or TCP( C ) for 24 h. The amount of the intracellular GGA represents the mean ± SD in triplicate. The IC 50 of ABT, BG or TCP was obtained using GraphPad Prism 9.3. GGA, geranylgeranoic acid; MAOB, monoamine oxidase B; ABT, 1-aminobenzotriazole; BG, bergamottin; TCP, tranylcypromine.

Article Snippet: Therefore, allosteric sigmoidal enzyme kinetics were selected in GraphPad Prism 9.3 software and the titration curve was approximated using the equation of Y = V m a x × X h ÷ K p r i m e + X h (r 2 = 0.9297), where the kinetics parameters were h = 1.598 and K prime = 3888 µM.

Techniques:

Journal: Metabolites

Article Title: Hepatic CYP3A4 Enzyme Compensatively Maintains Endogenous Geranylgeranoic Acid Levels in MAOB -Knockout Human Hepatoma Cells

doi: 10.3390/metabo12020140

Figure Lengend Snippet: Recombinant human CYP3A4 catalyzed oxidation of GGOH to GGal. The increasing concentrations of GGOH were incubated with recombinant hCYP3A4 (0.1 mg protein) with 5 mM NADPH at 37 °C for 1 h. The amounts of GGal products were measured by LC/MS/MS analysis. Each point represents the mean ± SD ( n = 3). The titration curve was drawn by allosteric sigmoidal enzyme kinetics in GraphPad Prism 9.3 software. GGal, geranylgeranial; GGOH; geranylgeraniol.

Article Snippet: Therefore, allosteric sigmoidal enzyme kinetics were selected in GraphPad Prism 9.3 software and the titration curve was approximated using the equation of Y = V m a x × X h ÷ K p r i m e + X h (r 2 = 0.9297), where the kinetics parameters were h = 1.598 and K prime = 3888 µM.

Techniques: Recombinant, Incubation, Liquid Chromatography with Mass Spectroscopy, Titration, Software